RESUMO
OBJECTIVE: To evaluate survivin gene expression in granulosa cells from infertile patients and examine the relationship between survivin gene expression and infertile clinical background. DESIGN: Prospective study. SETTING: IVF-ET program at Osaka Medical College. PATIENT(S): Twenty-eight patients underwent ovulation induction for IVF-ET performed because of tubal infertility, male factor infertility, or endometriosis. INTERVENTION(S): Granulosa cells obtained at oocyte retrieval were examined for survivin gene expression by quantitative reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURE(S): Hormone environment, number of oocytes, fertilization rate, high-quality embryo rate, pregnancy rate, and expression of survivin genes. RESULT(S): Survivin gene expression was detected in all granulosa cells. The gene expression levels of survivin in patients with endometriosis were significantly lower than those in patients with male factor infertility. The gene expression levels of survivin in total pregnant patients were higher than those in total nonpregnant patients and than those in the male factor infertility group, and there was no correlation between gene expression level and serum E(2) level. CONCLUSION(S): Survivin may be used as an indicator of the success of IVF-ET, and the existence of endometriosis may elevate the apoptosis of granulosa cells.
Assuntos
Transferência Embrionária , Fertilização in vitro , Regulação da Expressão Gênica no Desenvolvimento , Células da Granulosa/química , Infertilidade/terapia , Proteínas Associadas aos Microtúbulos/análise , Proteínas de Neoplasias/análise , Adulto , Apoptose , Biomarcadores/análise , Endometriose/complicações , Endometriose/genética , Endometriose/metabolismo , Endometriose/terapia , Estradiol/sangue , Doenças das Tubas Uterinas/complicações , Doenças das Tubas Uterinas/genética , Doenças das Tubas Uterinas/metabolismo , Doenças das Tubas Uterinas/terapia , Feminino , Fertilização , Células da Granulosa/patologia , Humanos , Infertilidade/etiologia , Infertilidade/genética , Infertilidade/metabolismo , Infertilidade/fisiopatologia , Infertilidade Masculina/genética , Infertilidade Masculina/metabolismo , Infertilidade Masculina/terapia , Proteínas Inibidoras de Apoptose , Masculino , Proteínas Associadas aos Microtúbulos/genética , Proteínas de Neoplasias/genética , Recuperação de Oócitos , Indução da Ovulação , Gravidez , Taxa de Gravidez , Estudos Prospectivos , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Survivina , Resultado do TratamentoRESUMO
The expression of mitochondrial genes in granulosa cells was quantitated by real-time polymerase chain reaction. The expression ratio of mitochondrial genes in granulosa cells of patients with severe endometriosis showed no statistically significant difference compared with cases of tubal infertility without endometriosis.
Assuntos
DNA Mitocondrial/metabolismo , Transferência Embrionária , Endometriose/genética , Fertilização in vitro , Células da Granulosa/metabolismo , Mitocôndrias/genética , Doenças Uterinas/genética , Endometriose/complicações , Endometriose/metabolismo , Feminino , Expressão Gênica , Humanos , Infertilidade Feminina/etiologia , Infertilidade Feminina/genética , Resultado do Tratamento , Doenças Uterinas/complicações , Doenças Uterinas/metabolismoRESUMO
Survivin is a novel inhibitor of apoptosis (IAP), and the two splice variants of survivin (survivin-2B and survivin-EX3) have been identified. Gene expression levels of survivin, survivin-2B and survivin-EX3 in 56 ectopic (16 peritoneal red and 16 peritoneal black lesions and 24 ovarian endometriomata) and 13 eutopic endometrial tissues surgically obtained from 42 women with endometriosis (group A) were compared with those in 16 control eutopic endometrium from 16 women without endometriosis (group B) by quantitative RT-PCR analysis. Survivin mRNA expression levels in ectopic endometriotic tissues were significantly higher than those in eutopic endometrium of groups A and B over the whole cycle. Red peritoneal lesions had higher gene expression levels of survivin than black lesions. In contrast, all tissue samples examined showed relatively lower gene expression levels of survivin-2B and survivin-EX3. No cyclic variation was found in survivin and the two splice variants, both in ectopic and in eutopic endometrium. Although there was no significant difference in the ratio of survivin-2B/survivin between ectopic and eutopic endometrium, the ratio of survivin-EX3/survivin in peritoneal endometriotic lesions was significantly higher than that of eutopic endometrium of groups A and B. These results suggest that survivin and survivin-EX3 may be closely linked to escape from apoptosis and the development of endometriosis.
